NAGK Protein (His tag)

Catalog No. ABIN1098243

This Recombinant NAGK protein is expressed in Escherichia coli (E. coli).

Quick Overview for NAGK Protein (His tag) (ABIN1098243)

Target: NAGK (N-Acetylglucosamine Kinase (NAGK))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Purification tag / Conjugate: This NAGK protein is labelled with His tag.

Purification: purified by chromatography

Application Details

Comment:

Synonyms: N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 20% glycerol, 200 mM NaCl

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: NAGK (N-Acetylglucosamine Kinase (NAGK))

Alternative Name: NAGK

Background: NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity. Recombinant human NAGK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 39.8kDa (367aa), confirmed by MALDI-TOF

NCBI Accession: NP_060037