ICT1 Protein (His tag)

Catalog No. ABIN1098472

Recombinant ICT1 protein expressed in Escherichia coli (E. coli).

Quick Overview for ICT1 Protein (His tag) (ABIN1098472)

Target: ICT1 (Immature Colon Carcinoma Transcript 1 (ICT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % by SDS - PAGE

Product Details

Purification tag / Conjugate: This ICT1 protein is labelled with His tag.

Purification: purified by chromatography

Application Details

Comment:

Synonyms: Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 30% glycerol, 1 MM DTT

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: ICT1 (Immature Colon Carcinoma Transcript 1 (ICT1))

Alternative Name: ICT1

Background: Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. It may be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Recombinant human ICT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 22.8 kDa (200aa) confirmed by MALDI-TOF

NCBI Accession: NP_001536