TBPL1 Protein (AA 1-186) (His tag)
Quick Overview for TBPL1 Protein (AA 1-186) (His tag) (ABIN1098505)
Target
See all TBPL1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-186
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Purification tag / Conjugate
- This TBPL1 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMDADSDV ALDILITNVV CVFRTRCHLN LRKIALEGAN VIYKRDVGKV LMKLRKPRIT ATIWSSGKII CTGATSEEEA KFGARRLARS LQKLGFQVIF TDFKVVNVLA VCNMPFEIRL PEFTKNNRPH ASYEPELHPA VCYRIKSLRA TLQIFSTGSI TVTGPNVKAV ATAVEQIYPF VFESRKEIL
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Purification
- purified by chromatography
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TBP-Like 1 (TBPL1) (AA 1-186) protein (His tag)
custom-made TBPL1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TBP-Like 1 (TBPL1) (AA 1-186) protein (His tag)custom-made TBPL1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TBP-Like 1 (TBPL1) (AA 1-186) protein (GST tag)TBPL1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
TBP-Like 1 (TBPL1) proteinTBPL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
TBP-Like 1 (TBPL1) (AA 1-185), (partial) protein (His-SUMO Tag)TBPL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 50% glycerol, 2 mM DTT, 1 MM EDTA
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- TBPL1 (TBP-Like 1 (TBPL1))
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Alternative Name
- TBPL1
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Background
- TBPL1, also known as STuD, TLF, TLP and TRF2, belongs to the TBP family. The TATA box-binding protein (TBP) is an essential component of the basal transcriptional machinery. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. It is essential for spermiogenesis and believed to be important in expression of developmentally regulated genes. Recombinant human TBPL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 23.3 kDa (209aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_004856
Target
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