HFE Protein (His tag)

Catalog No. ABIN1098722

This Recombinant HFE protein is produced in Escherichia coli (E. coli).

Quick Overview for HFE Protein (His tag) (ABIN1098722)

Target: HFE (Hemochromatosis (HFE))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 85 % by SDS - PAGE

Product Details

Purification tag / Conjugate: This HFE protein is labelled with His tag.

Application Details

Comment:

Synonyms: Hereditary hemochromatosis protein, HFE1, HH, HLA-H, MVCD7, TFQTL2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M Urea, 10% glycerol

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: HFE (Hemochromatosis (HFE))

Alternative Name: HFE

Background: HFE, also known as HLA-H, belongs to the MHC class I family. This protein contains 1 Ig-like C1-type (immunoglobulin-like) domain. HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta-2 microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor (TFR) with transferrin. It binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin. Recombinant human HFE protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 35.7kDa (308aa)

NCBI Accession: NP_000401

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process