Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) protein (His tag)

Details for Product No. ABIN1098754
Protein Name
Origin
Human
4
1
1
1
Source
Escherichia coli (E. coli)
2
2
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This BUB3 protein is labelled with His tag.
Application
SDS-PAGE (SDS)
Options
Purity > 85 % by SDS - PAGE
Protein Name
Background Mitotic checkpoint protein BUB3, also known as BUB3, is a conserved component of the mitotic spindle assembly complex (MCC). The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. Recombinant human BUB3 protein, fused to His-tag at N-terminus, was expressed in E.coli.
Molecular Weight 39.5 kDa (351aa)
NCBI Accession NP_004716
Pathways Maintenance of Protein Location
Comment

Synonyms: Mitotic checkpoint protein BUB3, BUB3L, hBUB3
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml (determined by Bradford assay)
Buffer 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M Urea, 10% glycerol
Storage 4 °C
Storage Comment Avoid repeated freezing and thawing cycles.
Images
Image no. 1 for Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) protein (His tag) (ABIN1098754) Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) protein (His tag)