BUB3 Protein (His tag)
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- Target See all BUB3 Proteins
- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BUB3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purity
- > 85 % by SDS - PAGE
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Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) protein (GST tag)
BUB3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-326) protein (Strep Tag)Crystallography grade BUB3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) protein (Strep Tag)Crystallography grade BUB3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)BUB3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Mitotic checkpoint protein BUB3, BUB3L, hBUB3
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M Urea, 10% glycerol
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
- Alternative Name
- BUB3 (BUB3 Products)
- Synonyms
- BUB3L Protein, hBUB3 Protein, Aa2-050 Protein, xbub3 Protein, AU019800 Protein, AU021329 Protein, AU043350 Protein, AW146323 Protein, C78067 Protein, BUB3, mitotic checkpoint protein Protein, BUB3 mitotic checkpoint protein Protein, BUB3 mitotic checkpoint protein L homeolog Protein, BUB3 Protein, Bub3 Protein, bub3.L Protein
- Background
- Mitotic checkpoint protein BUB3, also known as BUB3, is a conserved component of the mitotic spindle assembly complex (MCC). The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. Recombinant human BUB3 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 39.5 kDa (351aa)
- NCBI Accession
- NP_004716
- Pathways
- Maintenance of Protein Location
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