Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2715362
Protein Name
Protein Characteristics
Transcript Variant 2
2
2
1
Origin
Human
4
1
1
1
Source
HEK-293 Cells
2
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This BUB3 protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human BUB3 (transcript variant 2) protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Protein Name
Background This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Molecular Weight 36.8 kDa
NCBI Accession NP_001007794
Pathways Maintenance of Protein Location
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.
Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Images
Image no. 1 for Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag) (ABIN2715362) Validation with Western Blot