beta Actin Protein (AA 1-375) (GST tag)
(1 reference)-
- Target See all beta Actin (ACTB) Proteins
- beta Actin (ACTB) (Actin, beta (ACTB))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-375
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This beta Actin protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- ACTB (Human) Recombinant Protein (P01)
- Sequence
- MDDDIAALVV DNGSGMCKAG FAGDDAPRAV SPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERRYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF
- Characteristics
- Human ACTB full-length ORF ( AAH01301, 1 a.a. - 375 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Actin, beta (ACTB) (AA 14-167) protein (His tag)
ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Actin, beta (ACTB) (AA 102-375) protein (His tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Poly(ADP-ribose) polymerase 1 is indispensable for transforming growth factor-? Induced Smad3 activation in vascular smooth muscle cell." in: PLoS ONE, Vol. 6, Issue 10, pp. e27123, (2011) (PubMed).
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: "Poly(ADP-ribose) polymerase 1 is indispensable for transforming growth factor-? Induced Smad3 activation in vascular smooth muscle cell." in: PLoS ONE, Vol. 6, Issue 10, pp. e27123, (2011) (PubMed).
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- Target
- beta Actin (ACTB) (Actin, beta (ACTB))
- Alternative Name
- ACTB (ACTB Products)
- Background
-
Full Gene Name: actin, beta
Synonyms: PS1TP5BP1 - Gene ID
- 60
- Pathways
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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