LUM Protein (AA 1-338) (GST tag)

Catalog No. ABIN1309888

This Recombinant LUM protein is produced in Wheat germ. There are 2 publications available.

Quick Overview for LUM Protein (AA 1-338) (GST tag) (ABIN1309888)

Target: LUM (Lumican (LUM))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-338

Purification tag / Conjugate: This LUM protein is labelled with GST tag.

Purpose: LUM (Human) Recombinant Protein (P01)

Sequence: MSLSAFTLFL ALIGGTSGQY YDYDFPLSIY GQSSPNCAPE CNCPESYPSA MYCDELKLKS VPMVPPGIKY LYLRNNQIDH IDEKAFENVT DLQWLILDHN LLENSKIKGR VFSKLKQLKK LHINHNNLTE SVGPLPKSLE DLQLTHNKIT KLGSFEGLVN LTFIHLQHNR LKEDAVSAAF KGLKSLEYLD LSFNQIARLP SGLPVSLLTL YLDNNKISNI PDEYFKRFNA LQYLRLSHNE LADSGIPGNS FNVSSLVELD LSYNKLKNIP TVNENLENYY LEVNQLEKFD IKSFCKILGP LSYSKIKHLR LDGNRISETS LPPDMYECLR VANEVTLN

Characteristics: Human LUM full-length ORF ( NP_002336.1, 1 a.a. - 338 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Littlechild, Zhang, Tomich, Conrad: "Fibrinogen, riboflavin, and UVA to immobilize a corneal flap--molecular mechanisms." in: Investigative ophthalmology & visual science, Vol. 53, Issue 10, pp. 5991-6003, (2012) (PubMed).

Zhang, Conrad, Conrad: "Effects of ultraviolet-A and riboflavin on the interaction of collagen and proteoglycans during corneal cross-linking." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13011-22, (2011) (PubMed).

Target Details

Target: LUM (Lumican (LUM))

Alternative Name: LUM

Background: Full Gene Name: lumican
Synonyms: LDC,SLRR2D

Gene ID: 4060

NCBI Accession: NM_002345

Pathways: Glycosaminoglycan Metabolic Process