PPID Protein (AA 1-370) (GST tag)

Catalog No. ABIN1315874

Product Details

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Protein Type: Recombinant

Protein Characteristics: AA 1-370

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This PPID protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: PPID (Human) Recombinant Protein (P01)

Sequence: MSHPSPQAKP SNPSNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGE KPAKLCVIAE CGELKEGDDG GIFPKDGSGD SHPDFPEDAD IDLKDVDKIL LITEDLKNIG NTFFKSQNWE MAIKKYAEVL RYVDSSKAVI ETADRAKLQP IALSCVLNIG ACKLKMSNWQ GAIDSCLEAL ELDPSNTKAL YRRAQGWQGL KEYDQALADL KKAQGIAPED KAIQAELLKV KQKIKAQKDK EKAVYAKMFA

Characteristics: Human PPID full-length ORF ( NP_005029.1, 1 a.a. - 370 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Nguyen, Stevens, Kohr, Steenbergen, Sack, Murphy: "Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore." in: The Journal of biological chemistry, Vol. 286, Issue 46, pp. 40184-92, (2011) (PubMed).

McGee, Baines: "Complement 1q-binding protein inhibits the mitochondrial permeability transition pore and protects against oxidative stress-induced death." in: The Biochemical journal, Vol. 433, Issue 1, pp. 119-25, (2010) (PubMed).

Target Details

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Alternative Name: PPID (PPID Products)

Background: Full Gene Name: peptidylprolyl isomerase D
Synonyms: CYP-40,CYPD,MGC33096

Gene ID: 5481

NCBI Accession: NM_005038

Pathways: Nuclear Hormone Receptor Binding