RNF8 Protein (AA 1-485) (GST tag)

Catalog No. ABIN1318397

Product Details

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Protein Type: Recombinant

Protein Characteristics: AA 1-485

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This RNF8 protein is labelled with GST tag.

Application: ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)

Purpose: RNF8 (Human) Recombinant Protein (P01)

Sequence: MGEPGFFVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLRVYSI HQGDYIQLGV PLENKENAEY EYEVTEEDWE TIYPCLSPKN DQMIEKNKEL RTKRKFSLDE LAGPGAEGPS NLKSKINKVS CESGQPVKSQ GKGEVASTPS DNLDPKLTAL EPSKTTGAPI YPGFPKVTEV HHEQKASNSS ASQRSLQMFK VTMSRILRLK IQMQEKHEAV MNVKKQTQKG NSKKVVQMEQ ELQDLQSQLC AEQAQQQARV EQLEKTFQEE EQHLQGLEIA QGEKDLKQQL AQALQEHWAL MEELNRSKKD FEAIIQAKNK ELEQTKEEKE KMQAQKEEVL SHMNDVLENE LQCIICSEYF IEAVTLNCAH SFCSYCINEW MKRKIECPIC RKDIKSKTYS LVLDNCINKM VNNLSSEVKE RRIVLIRERK AKRLF

Characteristics: Human RNF8 full-length ORF ( AAH07517, 1 a.a. - 485 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Delker, Zhou, Strikoudis, Stebbins, Papavasiliou: "Solubility-based genetic screen identifies RING finger protein 126 as an E3 ligase for activation-induced cytidine deaminase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 3, pp. 1029-34, (2013) (PubMed).

Nakada, Tai, Panier, Al-Hakim, Iemura, Juang, ODonnell, Kumakubo, Munro, Sicheri, Gingras, Natsume, Suda, Durocher: "Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1." in: Nature, Vol. 466, Issue 7309, pp. 941-6, (2010) (PubMed).

Target Details

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Alternative Name: RNF8 (RNF8 Products)

Background: Full Gene Name: ring finger protein 8
Synonyms: FLJ12013,KIAA0646

Gene ID: 9025

Pathways: Production of Molecular Mediator of Immune Response