WT1 Protein (AA 1-302) (GST tag)
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- Target See all WT1 Proteins
- WT1 (Wilms Tumor 1 (WT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-302
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This WT1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- WT1 (Human) Recombinant Protein (P01)
- Sequence
- MEKGYSTVTF DGTPSYGHTP SHHAAQFPNH SFKHEDPMGQ QGSLGEQQYS VPPPVYGCHT PTDSCTGSQA LLLRTPYSSD NLYQMTSQLE CMTWNQMNLG ATLKGVAAGS SSSVKWTEGQ SNHSTGYESD NHTTPILCGA QYRMHTHGVF RGIQDVRRVP GVAPTLVRSA SETSEKRPFM CAYPGCNKRY FKLSHLQMHS RKHTGEKPYQ CDFKDCERRF SRSDQLKRHQ RRHTGVKPFQ CKTCQRKFSR SDHLKTHTRT HTGEKPFSCR WPSCQKKFAR SDELVRHHNM HQRNMTKLQL AL
- Characteristics
- Human WT1 full-length ORF ( AAH32861.1, 1 a.a. - 302 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
- Top Product
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Wilms Tumor 1 (WT1) (AA 1-449) protein (His tag)
custom-made WT1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Wilms Tumor 1 (WT1) (AA 157-445) protein (His tag)WT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Wilms Tumor 1 (WT1) (AA 349-439) protein (GST tag)WT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Wilms Tumor 1 (WT1) (AA 147-446), (partial) protein (His tag)WT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Wilms Tumor 1 (WT1) (AA 1-449), (full length) protein (His-SUMO Tag)WT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- WT1 (Wilms Tumor 1 (WT1))
- Alternative Name
- WT1 (WT1 Products)
- Background
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Full Gene Name: Wilms tumor 1
Synonyms: GUD,WAGR,WIT-2,WT33 - Gene ID
- 7490
- Pathways
- Tube Formation
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