TACI Protein (AA 2-118) (Fc Tag)

Catalog No. ABIN1344232

Product Details

Target: TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 2-118

Origin: Human, Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TACI protein is labelled with Fc Tag.

Application: SDS-PAGE (SDS)

Purpose: TACI (human):Fc (human) (rec.)

Specificity: Binds to human and mouse BAFF and APRIL.

Cross-Reactivity: Human, Mouse

Characteristics: The extracellular domain of human TACI (aa 2-118) is fused at the C-terminus to the Fc portion of human IgG1.

Purity: >90 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Inhibits BAFF and APRIL activity.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile water.

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Huard, Tran, Benkhoucha, Manzin-Lorenzi, Santiago-Raber: "Selective APRIL blockade delays systemic lupus erythematosus in mouse." in: PLoS ONE, Vol. 7, Issue 2, pp. e31837, (2012) (PubMed).

Target Details

Target: TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))

Alternative Name: TACI (TNFRSF13B Products)

Background:

TNFRSF 13B, Tumor Necrosis Factor Receptor Superfamily Member 13B, Transmembrane Activator and CAML interactor, CD267

B-cell activating factor belonging to the TNF family receptor (BAFF-R), B-cell maturation antigen (BCMA), and transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) play critical functions in promoting B-cell survival by engaging the ligands a proliferation-inducing ligand (APRIL) and/or BAFF. TACI is predominantly expressed on peripheral blood B cells and interacts with the B cell activating factor (BAFF) of ligands and a proliferation inducing ligand (APRIL), which are expressed by numerous cell types, including dendritic cells, macrophages and neutrophils. TNFRSF13B, the gene, which encodes TACI is mutated in nearly 10 % of patients with common variable immune deficiency (CVID), an antibody deficiency syndrome characterized by loss of memory B cells and plasma cells.

Molecular Weight: ~45kDa (SDS-PAGE)

UniProt: O14836