Angiopoietin 2 Protein (ANGPT2) (AA 19-496) (DYKDDDDK Tag)

Catalog No. ABIN1344329

Product Details

Target: Angiopoietin 2 (ANGPT2)

Protein Type: Recombinant

Protein Characteristics: AA 19-496

Origin: Human, Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This Angiopoietin 2 protein is labelled with DYKDDDDK Tag.

Application: SDS-PAGE (SDS)

Purpose: Angiopoietin-2 (human) (rec.)

Specificity: Binds to the human and mouse Tie-2 receptor.

Cross-Reactivity: Human, Mouse

Characteristics: Human angiopoietin-2 (aa 19-496) is fused at the N-terminus to a FLAG®-tag.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: Contains PBS with trehalose and Brij35.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Kim, Shin, Kim, Choi, Byun, Jeon, Suh, Kim: "Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells." in: Cardiovascular research, Vol. 72, Issue 3, pp. 394-402, (2006) (PubMed).

Target Details

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angiopoietin-2 (ANGPT2 Products)

Background:

Ang-2, Ang2, Angpt2

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context.

Molecular Weight: ~70kDa (SDS-PAGE)

UniProt: O15123

Pathways: RTK Signaling