Angiopoietin 2 Protein (ANGPT2) (AA 275-496) (His-Avi Tag)

Catalog No. ABIN7273860

Product Details

Target: Angiopoietin 2 (ANGPT2)

Protein Type: Recombinant

Protein Characteristics: AA 275-496

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Angiopoietin 2 protein is labelled with His-Avi Tag.

Purpose: Human ANGPT2/Angiopoietin-2 Protein

Sequence: Lys275-Phe496

Characteristics: Recombinant Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Lys275-Phe496.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ANGPT2, His Tag at 2μg/ml (100μl/well). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: ANGPT2 (ANGPT2 Products)

Synonyms: AGPT2 Protein, ANG2 Protein, Agpt2 Protein, Ang-2 Protein, Ang2 Protein, ang2 Protein, angpt2 Protein, ANGIOPOIETIN-2 Protein, ANG-2 Protein, ANGPT2 Protein, Angrp Protein, Rnase5b Protein, ANG3 Protein, C11orf2 Protein, C11orf3 Protein, FFR Protein, angiopoietin 2 Protein, angiopoietin 2b Protein, angiogenin, ribonuclease A family, member 2 Protein, VPS51, GARP complex subunit Protein, ANGPT2 Protein, Angpt2 Protein, angpt2b Protein, Ang2 Protein, VPS51 Protein

Background: Angiopoietin-2 (Ang-2, also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies..

Molecular Weight: 28.4 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling