AMBN Protein (AA 1-447) (GST tag)

Catalog No. ABIN1344966

This Recombinant AMBN protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for AMBN Protein (AA 1-447) (GST tag) (ABIN1344966)

Target: AMBN (Ameloblastin (Enamel Matrix Protein) (AMBN))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-447

Purification tag / Conjugate: This AMBN protein is labelled with GST tag.

Purpose: AMBN (Human) Recombinant Protein (P01)

Sequence: MSASKIPLFK MKDLILILCL LEMSFAVPFF PQQSGTPGMA SLSLETMRQL GSLQRLNTLS QYSRYGFGKS FNSLWMHGLL PPHSSLPWMR PREHETQQYE YSLPVHPPPL PSQPSLKPQQ PGLKPFLQSA AATTNQATAL KEALQPPIHL GHLPLQEGEL PLVQQQVAPS DKPPKPELPG VDFADPQGPS LPGMDFPDPQ GPSLPGLDFA DPQGSTIFQI ARLISHGPMP QNKQSPLYPG MLYVPFGANQ LNAPARLGIM SSEEVAGGRE DPMAYGAMFP GFGGMRPGFE GMPHNPAMGG DFTLEFDSPV AATKGPENEE GGAQGSPMPE ANPDNLENPA FLTELEPAPH AGLLALPKDD IPGLPRSPSG KMKGLPSVTP AAADPLMTPE LADVYRTYDA DMTTSVDFQE EATMDTTMAP NSLQTSMPGN KAQEPEMMHD AWHFQEP

Characteristics: Human AMBN full-length ORF ( NP_057603.1, 1 a.a. - 447 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Geng, White, Paine, Snead: "Protein Interaction between Ameloblastin and Proteasome Subunit ? Type 3 Can Facilitate Redistribution of Ameloblastin Domains within Forming Enamel." in: The Journal of biological chemistry, Vol. 290, Issue 34, pp. 20661-73, (2015) (PubMed).

Target Details

Target: AMBN (Ameloblastin (Enamel Matrix Protein) (AMBN))

Alternative Name: AMBN

Background: Full Gene Name: ameloblastin (enamel matrix protein)
Synonyms:

Gene ID: 258

NCBI Accession: NM_016519