BAK1 Protein (AA 1-211) (GST tag)

Catalog No. ABIN1346390

This Recombinant BAK1 protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for BAK1 Protein (AA 1-211) (GST tag) (ABIN1346390)

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP), ELISA

Product Details

Protein Characteristics: AA 1-211

Purification tag / Conjugate: This BAK1 protein is labelled with GST tag.

Purpose: BAK1 (Human) Recombinant Protein (P01)

Sequence: MASGQGPGPP RQECGEPALP SASEEQVAQD TEEVFRSYVF YRHQQEQEAE GVAAPADPEM VTLPLQPSST MGQVGRQLAI IGDDINRRYD SEFQTMLQHL QPTAENAYEY FTKIATSLFE SGINWGRVVA LLGFGYRLAL HVYQHGLTGF LGQVTRFVVD FMLHHCIARW IAQRGGWVAA LNLGNGPILN VLVVLGVVLL GQFVVRRFFK S

Characteristics: Human BAK1 full-length ORF ( NP_001179.1, 1 a.a. - 211 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Lindner, Concannon, Boukes, Cannon, Llambi, Ryan, Boland, Kehoe, McNamara, Murray, Kay, Hector, Green, Huber, Prehn: "Systems analysis of BCL2 protein family interactions establishes a model to predict responses to chemotherapy." in: Cancer research, Vol. 73, Issue 2, pp. 519-28, (2013) (PubMed).

Target Details

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Alternative Name: BAK1

Background: Full Gene Name: BCL2-antagonist/killer 1
Synonyms: BAK,BAK-LIKE,BCL2L7,CDN1,MGC117255,MGC3887

Gene ID: 578

NCBI Accession: NM_001188

Pathways: Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response