BAX Protein (AA 1-192) (GST tag)

Catalog No. ABIN1346446

Product Details

Target: BAX (BCL2-Associated X Protein (BAX))

Protein Type: Recombinant

Protein Characteristics: AA 1-192

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This BAX protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: BAX (Human) Recombinant Protein (P01)

Sequence: MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG LLSYFGTPTW QTATIFVAGV LTASLTIWKK MG

Characteristics: Human BAX full-length ORF ( AAH14175, 1 a.a. - 192 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Kim, Kim, Park, Hwang, Kim, Lee, Kang, Um: "Bcl-w promotes cell invasion by blocking the invasion-suppressing action of Bax." in: Cellular signalling, Vol. 24, Issue 6, pp. 1163-72, (2012) (PubMed).

Chattopadhyay, Marques, Yamashita, Peters, Smith, Desai, Williams, Sen: "Viral apoptosis is induced by IRF-3-mediated activation of Bax." in: The EMBO journal, Vol. 29, Issue 10, pp. 1762-73, (2010) (PubMed).

Wang, Carlsson, Basso, Zhu, Rousset, Rasola, Johansson, Blomgren, Mallard, Bernardi, Forte, Hagberg: "Developmental shift of cyclophilin D contribution to hypoxic-ischemic brain injury." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 8, pp. 2588-96, (2009) (PubMed).

Target Details

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX (BAX Products)

Background: Full Gene Name: BCL2-associated X protein
Synonyms: BCL2L4

Gene ID: 581

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response