CD40 Ligand Protein (CD40LG) (AA 116-261) (His-DYKDDDDK-Strep II Tag)
Quick Overview for CD40 Ligand Protein (CD40LG) (AA 116-261) (His-DYKDDDDK-Strep II Tag) (ABIN1348648)
Target
See all CD40 Ligand (CD40LG) ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 116-261
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Purification tag / Conjugate
- This CD40 Ligand protein is labelled with His-DYKDDDDK-Strep II Tag.
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Purpose
- CD40LG (Human) Recombinant Protein
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Sequence
- GDQNPQIAAH VISEASSKTT SVLQWAEKGY YTMSNNLVTL ENGKQLTVKR QGLYYIYAQV TFCSNREASS QAPFIASLCL KSPGRFERIL LRAANTHSSA KPCGQQSIHL GGVFELQPGA SVFVNVTDPS QVSHGTGFTS FGLLKL
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Characteristics
- Purified CD40LG (AAH74950.1, 116 a.a. - 261 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
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Purification
- Transfection of pSuper-CD40LG plasmid into HEK293T cell, and the expressed protein was purified by Strep-Tactin affinity column.
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CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (Fc Tag)
CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag,Biotin)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 1-260) protein (His tag)custom-made CD40LG Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 1-261) protein (His tag)custom-made CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 112-260), (Trimer) protein (Fc Tag)CD40LG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG Origin: Human, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) proteinCD40LG Origin: Human Host: Human Cells Recombinant >90 % as determined by reducing SDS-PAGE.
CD40 Ligand (CD40LG) (Active) protein (His tag)CD40LG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Transfection of pSuper-CD40LG plasmid into HEK293T cell, and the expressed protein was purified by Strep-Tactin affinity column.
Product Quality tested by: SDS-PAGE and Western Blot -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 10 μg/mL
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Buffer
- 100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
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Preservative
- Other preservative
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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- CD40 Ligand (CD40LG)
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Alternative Name
- CD40LG
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Background
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Full Gene Name: CD40 ligand
Synonyms: CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39,hCD40L -
Gene ID
- 959
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Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Target
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