EIF4G1 Protein (AA 1-645) (GST tag)
(1 reference)-
- Target See all EIF4G1 Proteins
- EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-645
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This EIF4G1 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)
- Purpose
- EIF4G1 (Human) Recombinant Protein (P01)
- Sequence
- MDQYFNQMEK IIKEKKTSSR IRFMLQDVLD LRGSNWVPRR GDQGPKTIDQ IHKEAEMEEH REHIKVQQLM AKGSDKRRGG PPGPPISRGL PLVDDGGWNT VPISKGSRPI DTSRLTKITK PGSIDSNNQL FAPGGRLSWG KGSSGGSGAK PSDAASEAAR PATSTLNRFS ALQQAVPTES TDNRRVVQRS SLSRERGEKA GDRGDRLERS ERGGDRGDRL DRARTPATKR SFSKEVEERS RERPSQPEGL RKAASLTEDR DRGRDAVKRE AALPPVSPLK AALSEEELEK KSKAIIEEYL HLNDMKEAVQ CVQELASPSL LFIFVRHGVE STLERSAIAR EHMGQLLHQL LCAGHLSTAQ YYQGLYEILE LAEDMEIDIP HVWLYLAELV TPILQEGGVP MGELFREITK PLRPLGKAAS LLLEILGLLC KSMGPKKVGT LWREAGLSWK EFLPEGQDIG AFVAEQKVEY TLGEESEAPG QRALPSEELN RQLEKLLKEG SSNQRVFDWI EANLSEQQIV SNTLVRALMT AVCYSAIIFE TPLRVDVAVL KARAKLLQKY LCDEQKELQA LYALQALVVT LEQPPNLLRM FFDALYDEDV VKEDAFYSWE SSKDPAEQQG KGVALKSVTA FFKWLREAEE ESDHN
- Characteristics
- Human EIF4G1 full-length ORF ( AAH07788, 1 a.a. - 645 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1250-1599) protein (His tag)
EIF4G1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1500-1599) protein (GST tag)EIF4G1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1-1599) protein (His tag)Crystallography grade EIF4G1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1-1600) protein (His tag)Crystallography grade EIF4G1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (Transcript Variant 5) protein (Myc-DYKDDDDK Tag)EIF4G1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1250-1599), (partial) protein (GST tag)EIF4G1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1250-1599), (partial) protein (His tag)EIF4G1 Origin: Human Host: Yeast Recombinant > 90 % SDS
Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)EIF4G1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Two-site phosphorylation of EPRS coordinates multimodal regulation of noncanonical translational control activity." in: Molecular cell, Vol. 35, Issue 2, pp. 164-80, (2009) (PubMed).
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: "Two-site phosphorylation of EPRS coordinates multimodal regulation of noncanonical translational control activity." in: Molecular cell, Vol. 35, Issue 2, pp. 164-80, (2009) (PubMed).
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- Target
- EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))
- Alternative Name
- EIF4G1 (EIF4G1 Products)
- Background
-
Full Gene Name: eukaryotic translation initiation factor 4 gamma, 1
Synonyms: DKFZp686A1451,EIF4F,EIF4G,p220 - Gene ID
- 1981
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