EIF4G1 Protein (Transcript Variant 5) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720102

Product Details

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 5

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This EIF4G1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human EIF4G1 / EIF4F (transcript variant 5) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Liberman, Gandin, Svitkin, David, Virgili, Jaramillo, Holcik, Nagar, Kimchi, Sonenberg: "DAP5 associates with eIF2β and eIF4AI to promote Internal Ribosome Entry Site driven translation." in: Nucleic acids research, Vol. 43, Issue 7, pp. 3764-75, (2015) (PubMed).

Target Details

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Alternative Name: Eif4g1,eif4f (EIF4G1 Products)

Background: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Molecular Weight: 154.6 kDa

NCBI Accession: NP_004944