PROX1 Protein (partial)
Quick Overview for PROX1 Protein (partial) (ABIN1589681)
Target
See all PROX1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- partial
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Purpose
- Prox-1 (fragment)
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Sequence
- MAEGLSLSLI KSECGDLQDM SEISPYSGSA MQEGLSPNHL KKAKLMFFYT RYPSSNMLKT YFSDVKFNRC ITSQLIKWFS NFREFYYIQM EKYARQAIND GVTSTEELSI TRDCELYRAL NMHYNKANDF EVPERFLEVA QITLREFFNA IIAGKDVDPS WKKAIYKVIC KLDSEVPEIF KSPNCLQELL HE
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Specificity
- Chromosomal location:1q32.2-q32.3
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Characteristics
- Length (aa):192
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Prospero Homeobox 1 (PROX1) (AA 1-737) protein (His tag)
custom-made PROX1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Prospero Homeobox 1 (PROX1) (AA 1-737) protein (His tag)custom-made PROX1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Prospero Homeobox 1 (PROX1) (AA 547-737) protein (His tag)PROX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Prospero Homeobox 1 (PROX1) (AA 354-737) protein (His tag)PROX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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Application Notes
- Control for Western Blotting. Biological activity not tested.
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Comment
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Cytokines & Growth Factors
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge vial prior to opening. The lyophilized Prox-1 should be reconstituted in water to a concentration not lower than 50 μg/mL.
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Buffer
- PBS
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted Prox-1 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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- PROX1 (Prospero Homeobox 1 (PROX1))
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Alternative Name
- Prox-1
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Background
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Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lympathic vessels from blood vessels. Prox1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells. The highly conserved C-terminal part of the homeobox transcription factor Prox1 was produced in E. coli. It was not tested for activity and can be used as positive control e.g. in Western analysis.
Synonyms: PROX1, W117m -
Molecular Weight
- 22.35 kDa
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Gene ID
- 5629
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NCBI Accession
- NM_002763, NP_002754
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UniProt
- Q92786
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Pathways
- Stem Cell Maintenance, Regulation of Muscle Cell Differentiation
Target
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