ISPD Protein (AA 1-224) (His tag)

Catalog No. ABIN1668584

This Recombinant ISPD protein is produced in Yeast.

Quick Overview for ISPD Protein (AA 1-224) (His tag) (ABIN1668584)

Target: ISPD (2-C-Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase (ISPD))

Protein Type: Recombinant

Origin: Saccharopolyspora erythraea

Source: Yeast

Application: ELISA

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-224

Purification tag / Conjugate: This ISPD protein is labelled with His tag.

Sequence: MSVVALVPAA GRGVRLGAGV PKALVPVAGE SLLSRAVRGL HDSGRVRHVV VAAPADEVPA VEAELASLRS FVHVVPGGAE RTDSVRLALA EAERVVPDAR VVLVHDAARA FTPPSVVRDV VRAVEEGAPA VVPVLPVADT IKQVDEAGDV ETTVDRSRLR TVQTPQGFAI DVLRQAYAAA GDIATDDAGL VERIGGKVST VPGHPHALKI TTAFDLAIAE AVLA

Specificity: Saccharopolyspora erythraea (strain NRRL 23338)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: ISPD (2-C-Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase (ISPD))

Background: Recommended name: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase.
EC= 2.7.7.60.
Alternative name(s): 4-diphosphocytidyl-2C-methyl-D-erythritol synthase MEP cytidylyltransferase.
Short name= MCT

UniProt: A4F6W3