CA5B Protein (AA 34-317) (His tag)

Catalog No. ABIN1690853

This Recombinant CA5B protein is produced in Escherichia coli (E. coli).

Quick Overview for CA5B Protein (AA 34-317) (His tag) (ABIN1690853)

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 34-317

Purification tag / Conjugate: This CA5B protein is labelled with His tag.

Purpose: Recombinant Human Carbonic Anhydrase 5B/CA5B (C-6His)

Sequence: MCSLYTCTYK TRNRALHPLW ESVDLVPGGD RQSPINIRWR DSVYDPGLKP LTISYDPATC LHVWNNGYSF LVEFEDSTDK SVIKGGPLEH NYRLKQFHFH WGAIDAWGSE HTVDSKCFPA ELHLVHWNAV RFENFEDAAL EENGLAVIGV FLKLGKHHKE LQKLVDTLPS IKHKDALVEF GSFDPSCLMP TCPDYWTYSG SLTTPPLSES VTWIIKKQPV EVDHDQLEQF RTLLFTSEGE KEKRMVDNFR PLQPLMNRTV RSSFRHDYVL NVQAKPKPAT SQATPLEHHH HHH

Characteristics: Recombinant Human Carbonic Anhydrase 5B/CA5B is produced with our E. coli expression system. The target protein is expressed with sequence (Cys34-Pro317) of Human CA5B.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Alternative Name: ca5b

Background: Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide. CAs is associated with many biological processes, including calcification, respiration, bone resorption, acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart, pancreas, kidney, placenta, lung, and skeletal muscle, but it is restricted to the liver. CA5B is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA-VA. CA5B is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin, and Foscarnet.
Alternative Names: Carbonic Anhydrase 5B Mitochondrial, Carbonate Dehydratase VB, Carbonic Anhydrase VB, CA-VB, CA5B

Molecular Weight: 33.77 kDa

UniProt: Q9Y2D0