ATF4 Protein (AA 1-351) (GST tag)

Catalog No. ABIN1345933

This Recombinant ATF4 protein is produced in Wheat germ. There is 1 publication available.

Quick Overview for ATF4 Protein (AA 1-351) (GST tag) (ABIN1345933)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-351

Purification tag / Conjugate: This ATF4 protein is labelled with GST tag.

Purpose: ATF4 (Human) Recombinant Protein (P01)

Sequence: MTEMSFLSSE VLVGDLMSPF DPSGLGAEES LGLLDDYLEV AKHFKPHGFS SDKAKAGSSE WLAVDGLVSP SNNSKEDAFS GTDWMLEKMD LKEFDLDALL GIDDLETMPD DLLTTLDDTC DLFAPLVQET NKQPPQTVNP IGHLPESLTK PDQVAPFTFL QPPPLSPGVL SSTPDHSFSL ELGSEVDITE GDRKPDYTAY VAMIPQCIKE EDTPSDNDSG ICMSPESYLG SPQHSPSTRG SPNRSLPSPG VLCGSARPKP YDPPGEKMVA AKVKGEKLDK KLKKMEQNKT AATRYRQKKR AEQEALTGEC KELEKKNEAL KERADSLAKE IQYLKDLIEE VRKARGKKRV P

Characteristics: Human ATF4 full-length ORF ( AAH08090, 1 a.a. - 351 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Zhang, Khachigian et al.: "Injury-induced platelet-derived growth factor receptor-alpha expression mediated by interleukin-1beta (IL-1beta) release and cooperative transactivation by NF-kappaB and ATF-4: IL-1beta facilitates ..." in: The Journal of biological chemistry, Vol. 284, Issue 41, pp. 27933-43, (2009) (PubMed).

Target Details

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background: Full Gene Name: activating transcription factor 4 (tax-responsive enhancer element B67)
Synonyms: CREB-2,CREB2,TAXREB67,TXREB

Gene ID: 468

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response