HIST4H4 Protein

Catalog No. ABIN1880095

Native HIST4H4 protein expressed in Pig.

Quick Overview for HIST4H4 Protein (ABIN1880095)

Target: HIST4H4 (Histone Cluster 4, H4 (HIST4H4))

Protein Type: Native

Origin: Pig

Source: Pig

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP), SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile PBS, pH 7.2 - pH 7.4.

Buffer: Supplied as lyophilized form in 50 mM TRIS, 200 mM NaCl

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details

Target: HIST4H4 (Histone Cluster 4, H4 (HIST4H4))

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element.

Pathways: Telomere Maintenance