Actin, beta (ACTB) (AA 1-375) protein (His tag)

Catalog No. ABIN1880098

Product Details

Protein Name:

beta Actin (ACTB)

Protein Type: Recombinant

Protein Characteristics: AA 1-375

Origin: Chicken, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rat, Sheep, Sus scrofa

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This beta Actin protein is labelled with His tag.

Application: Immunoprecipitation (IP), SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Sequence: MGHHHHHHSG SEF-MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF

Characteristics: Location of tag(s): N-terminal

Purity: > 95 %

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile PBS, pH 7.2 - pH 7.4.

Buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5 % trehalose, 0.05 % sarcosyl.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details

Protein Name:

beta Actin (ACTB)

Molecular Weight: 43.3 kDa

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction