Actin, beta (ACTB) (AA 1-375) protein (His tag) Protein
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- Target
- beta Actin (ACTB)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-375
- Origin
- Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Horse, Pig, Sheep
- Source
-
Escherichia coli (E. coli)
- Purification tag / Conjugate
- This beta Actin protein is labelled with His tag.
- Application
- Immunoprecipitation (IP), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
- Sequence
- MGHHHHHHSG SEF-MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF
- Characteristics
- Location of tag(s): N-terminal
- Purity
- > 95 %
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Actin, beta (ACTB) (AA 1-375) protein (GST tag) Protein
ACTB Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Actin, beta (ACTB) (AA 1-375) protein (His tag) ProteinACTB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Actin, beta (ACTB) (AA 2-375) protein (His tag) ProteinACTB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Actin, beta (ACTB) (AA 1-375) protein (His tag) ProteinACTB Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Actin, beta (ACTB) (AA 2-375) protein (His tag) ProteinACTB Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Actin, beta (ACTB) protein (His tag) ProteinACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD
Actin, beta (ACTB) (AA 102-375), (partial) protein (His tag) ProteinACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Actin, beta (ACTB) protein (Myc-DYKDDDDK Tag) ProteinACTB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD
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- Application Notes
-
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in sterile PBS, pH 7.2 - pH 7.4.
- Buffer
- Supplied as lyophilized form in PBS, pH 7.4, containing 5 % trehalose, 0.05 % sarcosyl.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
-
- Target
- beta Actin (ACTB)
- Synonyms
- BRWS1, PS1TP5BP1, ACTA1, Actx, E430023M04Rik, beta-actin, ps1tp5bp1, act-2, actb, B-actin, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Bap47, CG4027, Dmel\\CG4027, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, Bact, ACT-5, ACTB, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05, Beta-actin, actin beta, actin, beta, cytoplasmic actin OlCA1, beta-actin, Actin 5C, actin gamma 1, actin, beta 1, actin, beta L homeolog, beta actin, actin, cytoplasmic 1, ACTB, Actb, actb, LOC100174883, LOC100304816, Act5C, ACTG1, actb1, actb.L, LOC100136352, LOC109073280
- Molecular Weight
- 43.3 kDa
- UniProt
- P60709
- Pathways
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
