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Actin, beta (ACTB) (AA 1-375) protein (His tag) Protein

ACTB Origin: Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Horse, Pig, Sheep Host: Escherichia coli (E. coli) Recombinant > 95 % IP, SDS, ELISA, WB
Catalog No. ABIN1880098
$438.00
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  • Target
    beta Actin (ACTB)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-375
    Origin
    Human, Mouse, Rat, Chicken, Cow, Dog, Guinea Pig, Horse, Pig, Sheep
    Source
    Escherichia coli (E. coli)
    • 25
    • 4
    • 4
    • 1
    • 1
    Purification tag / Conjugate
    This beta Actin protein is labelled with His tag.
    Application
    Immunoprecipitation (IP), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
    Sequence
    MGHHHHHHSG SEF-MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF
    Characteristics
    Location of tag(s): N-terminal
    Purity
    > 95 %
  • Application Notes
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute in sterile PBS, pH 7.2 - pH 7.4.
    Buffer
    Supplied as lyophilized form in PBS, pH 7.4, containing 5 % trehalose, 0.05 % sarcosyl.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    beta Actin (ACTB)
    Synonyms
    BRWS1, PS1TP5BP1, ACTA1, Actx, E430023M04Rik, beta-actin, ps1tp5bp1, act-2, actb, B-actin, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Bap47, CG4027, Dmel\\CG4027, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, Bact, ACT-5, ACTB, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05, Beta-actin, actin beta, actin, beta, cytoplasmic actin OlCA1, beta-actin, Actin 5C, actin gamma 1, actin, beta 1, actin, beta L homeolog, beta actin, actin, cytoplasmic 1, ACTB, Actb, actb, LOC100174883, LOC100304816, Act5C, ACTG1, actb1, actb.L, LOC100136352, LOC109073280
    Molecular Weight
    43.3 kDa
    UniProt
    P60709
    Pathways
    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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