Cathepsin D Protein (CTSD) (AA 21-410) (His tag)

Catalog No. ABIN1880638

Recombinant Cathepsin D protein expressed in Human Cells.

Quick Overview for Cathepsin D Protein (CTSD) (AA 21-410) (His tag) (ABIN1880638)

Target: Cathepsin D (CTSD)

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-410

Purification tag / Conjugate: This Cathepsin D protein is labelled with His tag.

Purpose: Recombinant Mouse Cathepsin D/CSTD (C-6His)

Sequence: IIRIPLRKFT SIRRTMTEVG GSVEDLILKG PITKYSMQSS PKTTEPVSEL LKNYLDAQYY GDIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KILDIACWVH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC KSDQSKARGI KVEKQIFGEA TKQPGIVFVA AKFDGILGMG YPHISVNNVL PVFDNLMQQK LVDKNIFSFY LNRDPEGQPG GELMLGGTDS KYYHGELSYL NVTRKAYWQV HMDQLEVGNE LTLCKGGCEA IVDTGTSLLV GPVEEVKELQ KAIGAVPLIQ GEYMIPCEKV SSLPTVYLKL GGKNYELHPD KYILKVSQGG KTICLSGFMG MDIPPPSGPL WILGDVFIGS YYTVFDRDNN RVGFANAVVL VDHHHHHH

Characteristics: Recombinant Mouse Cathepsin D is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ile21-Leu410) of Mouse Cathepsin D fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM MES,150 mM NaCl, pH 5.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D

Sub Type: Fusionprotein

Background: CTSD localizes to the lysosome and consists of a light chain and a heavy chain. CTSD is expressed in epithelial cells as well as in macrophages.CTSD is a lysosomal aspartyl protease that depends critically on protonation of its active site Asp residue and gets activated at pH 5 in endosome of hepatocytes. It has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix and xonnective tissue. In addition, CTSD has been used as a breast cancer tumor marker.
Alternative Names: Cathepsin D, also known as CTSD and CPSD, is a member of the peptidase A1 family.

Molecular Weight: 43.9 kDa

UniProt: P18242

Pathways: Peptide Hormone Metabolism