PGK1 Protein (AA 2-417) (His tag)

Catalog No. ABIN1945463

This Recombinant PGK1 protein is expressed in Human Cells.

Quick Overview for PGK1 Protein (AA 2-417) (His tag) (ABIN1945463)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-417

Purification tag / Conjugate: This PGK1 protein is labelled with His tag.

Purpose: Recombinant Human Phosphoglycerate Kinase 1/PGK1 (C-6His)

Sequence: SLSNKLTLDK LDVKGKRVVM RVDFNVPMKN NQITNNQRIK AAVPSIKFCL DNGAKSVVLM SHLGRPDGVP MPDKYSLEPV AVELKSLLGK DVLFLKDCVG PEVEKACANP AAGSVILLEN LRFHVEEEGK GKDASGNKVK AEPAKIEAFR ASLSKLGDVY VNDAFGTAHR AHSSMVGVNL PQKAGGFLMK KELNYFAKAL ESPERPFLAI LGGAKVADKI QLINNMLDKV NEMIIGGGMA FTFLKVLNNM EIGTSLFDEE GAKIVKDLMS KAEKNGVKIT LPVDFVTADK FDENAKTGQA TVASGIPAGW MGLDCGPESS KKYAEAVTRA KQIVWNGPVG VFEWEAFARG TKALMDEVVK ATSRGCITII GGGDTATCCA KWNTEDKVSH VSTGGGASLE LLEGKVLPGV DALSNILDHH HHHH

Characteristics: Recombinant Human PGK1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (AA 2-417) of Human PGK1 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl,20 % glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: Phosphoglycerate Kinase 1/Cell Migration-Inducing Gene 10 Protein

Background: PGK1 is called phosphoglycerate kinase that involved in a critical energy-producing process known as glycolysis. Phosphoglycerate kinase helps carry out a chemical reaction that converts a molecule called 1,3-diphosphoglycerate, which is produced during the breakdown of glucose, to another molecule called 3-phosphoglycerate during glycolysis. PGK1 The encoded protein may also act as a cofactor for polymerase alpha.. The protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.

Molecular Weight: 45.5 kDa

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process