beta 2 Defensin Protein

Catalog No. ABIN2017676

Product Details

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Sequence: AVGSLKSIGY EAELDHCHTN GGYCVRAICP PSARRPGSCF PEKNPCCKYM K

Characteristics: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration of 10-100 ng/mL.

Purity: > 98 % by SDS-PAGE and HPLC analyses.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1 EU/μg of rMuBD-2 as determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Target Details

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Alternative Name: beta Defensin-2(BD-2) (BD-2 Products)

Background: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four beta-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
Synonyms: BD 2 Murine , BD-2 Murine

Molecular Weight: 5.5 kDa, a single non-glycosylated polypeptide chain containing 51 amino acids.