HGF Protein (AA 32-494, AA 495-728)
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- Target See all HGF Proteins
- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 32-494, AA 495-728
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Origin
- Human
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Source
- CHO Cells
- Characteristics
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ED50 < 10 ng/mL, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1x10^5 units/mg.
Alpha chain: AA 32-494, beta chain: AA 495-728 - Purity
- > 95 % as analyzed by SDS-PAGE.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product HGF Protein
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Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) protein (His tag)
HGF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) protein (Fc Tag)HGF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 496-728) protein (His-IF2DI Tag)HGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 493-721) protein (His-IF2DI Tag)HGF Origin: Chicken Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 619-728) protein (GST tag)HGF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) (Active) protein (His tag)HGF Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) (Active) protein (Fc Tag)HGF Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 477-728) protein (GST tag)HGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant 90 % SDS, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)HGF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O or PBS at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant humanHepatocyte Growth Factor (HGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, human Hepatocyte Growth Factor (HGF) should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
- Alternative Name
- Hepatocyte Growth Factor (HGF) (HGF Products)
- Background
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Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.Recombinant human Hepatocyte Growth Factor (rhHGF) is produced in CHO cells and consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in the formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 234 amino acid residues. A fully biologically active molecule, rhHGF has a molecular mass of around 88-90 kDa analyzed by reducing SDS-PAGE.
Synonyms: - Molecular Weight
- 88-90 kDa (single chain), 59-61kDa (alpha chain), 30-34kDa (beta chain), observed by reducing SDS-PAGE.
- Pathways
- RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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