Long R3 Insulin Like Growth Factor-1 (Active) Protein

Catalog No. ABIN2018201

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for Long R3 Insulin Like Growth Factor-1 (Active) Protein (ABIN2018201)

Target: Long R3 Insulin Like Growth Factor-1

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE.

Product Details

Sequence: MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Characteristics: ED50 < 10 ng/mL, measured by the dose-dependantproliferation of CHO cells, corresponding to a specific activity of >1.0x 10^5 units/mg.

Endotoxin Level: < 1 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in 10 mM HCl at 1 mg/mL. Use a 0.22 μm membrane for filter Sterilization.

Buffer: Lyophilized after dissolved in 48 % acetonitrile and 0.1 % TFA

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Long R3Insulin-like Growth Factor-1 (rhLR3IGF-1) remains stable up to 6 months at -80 °C from date of receipt. Stock solution of peptide can be stored at least 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: Long R3 Insulin Like Growth Factor-1

Background: IGF-1 is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-1 is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-2, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-1 (rhLR3IGF-1) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3)and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs. RhLR3IGF-1 is properly folded under oxidizing conditions and then purified by proprietary chromatographic techniques.
Synonyms: Human LR3 Insulin-like Growth Factor-I (LR3-IGF-I) (Receptor Grade)

Molecular Weight: 9.1±0.9 KDa, observed by reducing SDS-PAGE.