CCL2 Protein (AA 24-96)

Catalog No. ABIN2018226

This Recombinant CCL2 protein is expressed in HEK-293T Cells.

Quick Overview for CCL2 Protein (AA 24-96) (ABIN2018226)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293T Cells

Purity: > 98 % as analyzed by SDS-PAGE.

Product Details

Protein Characteristics: AA 24-96

Characteristics: The EC50 value of mouse MCP-1/CCL2 on Ca2+ mobilization assay in CHO-K1/Ga15/mCCR2 cells (human Ga15 and mouse CCR2 stably expressed in CHO-K1 cells) is less than 0.3 μg/mL.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant Mouse MCP-1/CCL2 remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, Mouse MCP-1/CCL2 should be stable up to 1 week at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP-1/CCL2

Background: Chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemotactic protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. CCL2 is implicated in the pathogeneses of several types of disease characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. CCL2 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. CCL2 is primarily secreted by monocytes, macrophages and dendritic cells. CCL2 can signal through the CCR2 receptor.Recombinant mouse MCP-1/CCL2 produced in HEK293 cells is a polypeptide chain containing 73 amino acids. A fully biologically active molecule, r mMCP-1/CCL2 has a molecular mass of 8 kDa analyzed by reducing SDS-PAGE.
Synonyms: MCP-1, MCAF, JE, Monocyte Chemotactic Protein-1 (MCP-1), CCL2

Molecular Weight: 8 kDa, observed by reducing SDS-PAGE.

UniProt: Q5SVU3

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection