LCP2 Protein (AA 1-533) (His tag,T7 tag)

Catalog No. ABIN2115473

Recombinant LCP2 protein expressed in Escherichia coli (E. coli).

Quick Overview for LCP2 Protein (AA 1-533) (His tag,T7 tag) (ABIN2115473)

Target: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-533

Purification tag / Conjugate: This LCP2 protein is labelled with His tag,T7 tag.

Purpose: Recombinant Human Lymphocyte Cytosolic Protein 2/LCP2 (C-6His, N-T7 tag)

Sequence: MASMTGGQQM GRGSMALRNV PFRSEVLGWD PDSLADYFKK LNYKDCEKAV KKYHIDGARF LNLTENDIQK FPKLRVPILS KLSQEINKNE ERRSIFTRKP QVPRFPEETE SHEEDNGGWS SFEEDDYESP NDDQDGEDDG DYESPNEEEE APVEDDADYE PPPSNDEEAL QNSILPAKPF PNSNSMYIDR PPSGKTPQQP PVPPQRPMAA LPPPPAGRNH SPLPPPQTNH EEPSRSRNHK TAKLPAPSID RSTKPPLDRS LAPFDREPFT LGKKPPFSDK PSIPAGRSLG EHLPKIQKPP LPPTTERHER SSPLPGKKPP VPKHGWGPDR RENDEDDVHQ RPLPQPALLP MSSNTFPSRS TKPSPMNPLP SSHMPGAFSE SNSSFPQSAS LPPYFSQGPS NRPPIRAEGR NFPLPLPNKP RPPSPAEEEN SLNEEWYVSY ITRPEAEAAL RKINQDGTFL VRDSSKKTTT NPYVLMVLYK DKVYNIQIRY QKESQVYLLG TGLRGKEDFL SVSDIIDYFR KMPLLLIDGK NRGSRYQCTL THAAGYPLEH HHHHH

Characteristics: Recombinant Human LCP2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Pro533) of Human LCP2 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl,20 % glycerol, pH 8.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Alternative Name: Lymphocyte Cytosolic Protein 2/LCP2

Background: Lymphocyte cytosolic protein 2(LCP2)contains a SAM domain and a SH2 domain. It is highly expressed in spleen, thymus and peripheral blood leukocytes, T-cell and monocytic cell lines, but expressed at lower level in B-cell lines. LCP2 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. It is phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2 during post-translational modification. Studies using LCP2-deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.
Synonyms: Lymphocyte cytosolic protein 2,SH2 domain-containing leukocyte protein of 76 kDa,SLP-76 tyrosine phosphoprotein,SLP76,LCP2

Molecular Weight: 62.6 kDa

UniProt: Q13094

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway