Human Immunodeficiency Virus Surface Glycoprotein (HIV gp120) (AA 36-511) protein (His tag)

Catalog No. ABIN2181174

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for Human Immunodeficiency Virus Surface Glycoprotein (HIV gp120) (AA 36-511) protein (His tag) (ABIN2181174)

Target: Human Immunodeficiency Virus Surface Glycoprotein (HIV gp120)

Protein Type: Recombinant

Origin: Human Immunodeficiency Virus (HIV)

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 36-511

Purification tag / Conjugate: His tag

Sequence: AA 36-511

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 54.1 kDa. The protein migrates as 65-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Target Details

Target: Human Immunodeficiency Virus Surface Glycoprotein (HIV gp120)

Alternative Name: GP120 (HIV)

Target Type: Viral Protein

Background: Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses. Envelope glycoprotein GP120 (or gp120) is the name of the glycoprotein which forms the spikes sticking out of a HIV virus particle. gp120 is essential for virus entry into cells as it plays a vital role in seeking out specific cell surface receptors for entry. Three gp120s, bound as heterodimers to a transmembrane glycoprotein, gp41, are thought to combine in a trimer to form the envelope spike, which is involved in virus-cell attachment. One half of the molecular weight of gp120 is due to the carbohydrate side chains (the "glyco-" in "glycoprotein"). These are sugar residues which form something almost like a sugar "dome" over the gp120 spikes. This dome prevents gp120 from being recognised by the human immune response. As the HIV virus and the human CD4 cell come together, the gp120 binding site "snaps open" at the last minute.The glycoprotein gp120 is anchored to the viral membrane, or envelope, via non-covalent bonds with the transmembrane glycoprotein, gp41. It is involved in entry into cells by binding to CD4 receptors, particularly helper T-cells. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.

Molecular Weight: 54.1 kDa

Gene ID: 17

UniProt: Q4QX31