RuvC (Active) Protein

Details for Product No. ABIN2452186, Supplier: Log in to see
Protein Name
Escherichia coli (E. coli)
Escherichia coli (E. coli)
Protein Type
Biological Activity
Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
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Characteristics Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
Background E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
UniProt P0A814
Application Notes 1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
2) SDS-PAGE (0. 2 g/lane)
3) Standard antigen for western blotting and ELISA
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
Storage -20 °C/-80 °C
Storage Comment Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
Supplier Images
SDS-PAGE (SDS) image for RuvC (Active) protein (ABIN2452186) RuvC (Active) protein
SDS-PAGE (SDS) image for RuvC (Active) protein (ABIN2452186) RuvC (Active) protein (Image 2)
Product cited in: Murayama, Kurokawa, Mayanagi, Iwasaki: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, 2008 (PubMed).

Shinagawa, Iwasaki: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, 1996 (PubMed).

Iwasaki, Takahagi, Shiba, Nakata, Shinagawa: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." in: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, 1992 (PubMed).

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