RuvC Protein (AA 2-173) (His-SUMO Tag)
RuvC
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS
1 image
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- Target See all RuvC products
- RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-173
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RuvC protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- AIILGIDPGS RVTGYGVIRQ VGRQLSYLGS GCIRTKVDDL PSRLKLIYAG VTEIITQFQP DYFAIEQVFM AKNADSALKL GQARGVAIVA AVNQELPVFE YAARQVKQTV VGIGSAEKSQ VQHMVRTLLK LPANPQADAA DALAIAITHC HVSQNAMQMS ESRLNLARGR LR
- Purification
- SDS-PAGE
- Purity
- > 90 %
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Crossover Junction Endodeoxyribonuclease RuvC (RuvC) (Active) protein
RuvC Origin: E. coli Host: Escherichia coli (E. coli) Native SDS, Func, ELISA, WB Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
- Alternative Name
- RUVC (RuvC Products)
- Synonyms
- ECK1864 Protein, JW1852 Protein, Crossover junction endodeoxyribonuclease RuvC Protein, ruvC Protein
- Background
- Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.
- Molecular Weight
- 34.62 kDa
- UniProt
- P0A814
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