TGF-beta Protein (TGFb)

Catalog No. ABIN2469123

This Recombinant TGF-beta protein is expressed in Hi-5 Cells.

Quick Overview for TGF-beta Protein (TGFb) (ABIN2469123)

Target: TGF-beta (TGFb)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Hi-5 Cells

Purity: < 98 % by SDS-PAGE gel and HPLC analyses.

Product Details

Sequence: ALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS

Characteristics: The ED50 was determined by its ability to inhibit the mouse IL - 4 - dependent proliferation of mouse HT - 2 cells. The ED50 was found to be < 0.2 ng/mL, corresponding to a specific activity of > 5 x 10⁶ units/mg.

Endotoxin Level: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: As with any protein, exposing TGF Beta recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -20 °C

Storage Comment: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted TGF Beta is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Expiry Date: 24 months

Target Details

Target: TGF-beta (TGFb)

Alternative Name: TGF beta

Background: The three mammalian isoforms of TGF-beta, TGF-beta1, beta2, beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development. Recombinant human TGF-beta2 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Gene ID: 7042

NCBI Accession: NP_001129071

OMIM: 208022653

UniProt: P61812

Pathways: EGFR Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus