Osteopontin Protein (AA 17-300, C-Term)

Catalog No. ABIN2666563

This Recombinant Osteopontin protein is produced in HEK-293 Cells.

Quick Overview for Osteopontin Protein (AA 17-300, C-Term) (ABIN2666563)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Western Blotting (WB)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: C-Term, AA 17-300

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 1.0 EU per μg of protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Human Osteopontin, when immobilized with anti-CD3 antibody, is able to inhibit IFN-γ secretion in human T cells in a dose-dependent manner with an ED50 of 0.5 - 2.0 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: Osteopontin

Background: Osteopontin, also known as secreted phosphoprotein 1 (SPP1) and early T lymphocytes activation 1 (ETA-1), is an arginine-glycine-aspartic acid (RGD) that contains multi-functional glycoproteins who are able to interact with integrins and CD44 as receptors. This protein has been shown to play roles in cell migration and survival, bone remodeling, regulation of immune cell functions, apoptosis, control of tumor cell phenotypes, and autoimmune diseases. Osteopontin is involved in recruiting and stimulating macrophages and lymphocytes in response to microbial infections. The Osteopontin receptor-ligand interaction mediates chemotaxis or attachment that depends on whether the protein is in soluble or immobilized form. Osteopontin has also been shown to play a role in the progression of cancer, which is supported by the finding that patients who suffer from disseminated carcinoma also have increased levels of Osteopontin in their plasma. In addition, Osteopontin is a component of the human atherosclerotic plaque and may be a mediator of arterial neointima formation. It is synthesized by macrophage, smooth muscle, and endothelial cells in primary and recurrent human coronary atherosclerotic plaques, which may contribute to the cellular accumulations and dystrophic calcification in the atherosclerotic plaques. Osteopontin is also a substrate for MMP-3, MMP-7, MMP-9, MMP-12, and thrombin within the SVVYGLR motif.

Molecular Weight: The 290 amino acid recombinant protein has a predicted molecular mass of approximately 33 kDa. The DTT-reduced and non-reduced protein migrates approximately 55 kDa by SDS-PAGE. The predicted N-terminal amino acid is Ile.

Pathways: Regulation of Cell Size