CXCL12 Protein (AA 22-89)

Catalog No. ABIN2666583

Recombinant CXCL12 protein expressed in Escherichia coli (E. coli).

Quick Overview for CXCL12 Protein (AA 22-89) (ABIN2666583)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 22-89

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract activated T cells in a dose dependent manner. The ED50 is 10 - 50 ng/ml, corresponding to a specific activity of 0.2 - 1.0 x 105 units/mg.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12

Background: Mouse cDNA CXCL12 was initially cloned from a bone marrow stromal cell library. CXCL12 and its receptor CXCR4 are involved in the regulation of migration, survival, and development of multiple cell types, including human hematopoietic CD34+/CD38-/low and stromal STRO-1+ stem cells. Stress-induced modulations in CXCL12 and CXCR4 levels participate in recruitment of immature and maturing leukocytes from the BM reservoir to damaged organs as part of the host defense and repair mechanism. In addition, CXCL12 and CXCR4 are key regulators of the stem cell mobilization process to ischemic tissues and therefore crucial for tissue repair. The CXCL12/CXCR4 system is involved in the establishment of organ metastasis in different cancers. Cancer stem cells (CSCs), a small subset of cancer cells, constitute a reservoir of self-sustaining cells with the ability to maintain tumor growth. Most of them express the CXCR4 receptor and respond to a chemotactic gradient of CXCL12, suggesting that CSCs probably represent a subpopulation capable of initiating metastasis. Additional data has shown that CXCL12 binds to a second receptor, CXCR7. This receptor is expressed in endothelial cells and is associated with tumors. CXCL12/CXCR4/CXCR7 axis activation seems to regulate the metastatic process.

Molecular Weight: The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7.9 kDa. The DTT-reduced protein migrates at approximately 10 kDa and the non-reduced protein migrates at approximately 12 kDa by SDS-PAGE. The N-terminal amino acid is

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling