CCL8 Protein (AA 24-97)

Catalog No. ABIN2666620

This Recombinant CCL8 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL8 Protein (AA 24-97) (ABIN2666620)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 24-97

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract human acute monocytic leukemia THP-1 cells in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: CCL8

Background: CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells stimulated with IL-1β. CCL8 (MCP-2) has structural and functional similarity with MCP-1 and MCP-3, and it is often co-induced with those chemokines in mononuclear cells. IFNγ is a strong inducer of CCL8 in monocytes and fibroblasts. CCL8 can be processed and secreted as a truncated chemokine CCL8 (6-75). The truncated isoform does not chemoattract monocytes, but it binds to the CCR2 receptor and induces its internalization, as a result, CCL8 inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of Th2 cell homing, which drives IL-5-mediated chronic allergic inflammation. In addition, CCL8 binds CCR5 receptor with high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 gene expression (by splenic stromal cells) is potentially relevant to the regulation of dendritic cell (DC) development. In addition, CCL8 expression is induced in splenic stromal cells during L. donovani infection and, in conjuction with CXCL12, hematopoietic stem and progenitor cells (HSPCs) are chemoattracted and potentially differentiated into regulatory DCs. Finally, CCL8 is a potential biomarker for the diagnosis of graft-versus-host diseases, tuberculosis, and sepsis.

Molecular Weight: The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.48 kDa. The DTT-reduced protein migrates at approximately 11 kDaa and non-reduced protein migrates at approximately 12 kDa by SDS-PAGE. The N-terminal amino acid is Gl