CXCL12 Protein (AA 22-89)

Catalog No. ABIN2666699

This Recombinant CXCL12 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CXCL12 Protein (AA 22-89) (ABIN2666699)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Purity: Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 22-89

Sterility: 0.22 μm filtered

Endotoxin Level:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract resting human T cells in a dose dependent manner. The ED 50 is 80 - 120 ng/ml, corresponding to a specific activity of 1.25-0.83 x 104 units/mg.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22μm filtered protein solution is in 20 mM Tris pH 8.0, 0.5M NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12

Background: Human SDF-1 belongs to the CXCL chemokine family. The mouse cDNA SDF was initially cloned from a bone marrow stromal cell library, and the human was cloned from a pro-B-cell cDNA library. SDF is expressed by many organs, and it is most abundantly expressed in pancreas, spleen, ovary and small intestine. SDF-1 (CXCL12) and its receptor CXCR4 are involved in regulation of migration, survival, and development of multiple cell types, including human hematopoietic CD34+/CD38-/low and stromal STRO-1+ stem cells. Stress-induced modulations in SDF-1 and CXCR4 levels participate in recruitment of immature and maturing leukocytes from the BM reservoir to damaged organs as part of host defense and repair mechanism. SDF-1 (CXCL12)/CXCR4 system is involved in the establishment of organ metastasis in different cancers, for example in lymph node metastasis in breast cancer and oral squamous cell carcinoma (SCC), and peritonel metastasis in ovarian cancer. Recently, several studies have demonstrated the existence of a small subset of cancer cells which share many characteristics with stem cells and named cancer stem cells (CSC). They constitute a reservoir of self-sustaining cells with the ability to maintain the tumor growth. Most of them express CXCR4 receptor and respond to a chemotactic gradient of its specific ligand SDF-1, suggesting that CSC probably represent a subpopulation capable of initiating metastasis.

Molecular Weight: The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7963.4 Da. The DTT-reduced protein migrates at approximately 7kDa and the non-reduced protein migrates at approximately 9kDa by SDS-PAGE. The N-terminal amino acid is Ly

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling