Lipocalin 2 Protein (LCN2) (AA 21-198, C-Term, His10)

Catalog No. ABIN2666721

Recombinant Lipocalin 2 protein expressed in HEK-293 Cells.

Quick Overview for Lipocalin 2 Protein (LCN2) (AA 21-198, C-Term, His10) (ABIN2666721)

Target: Lipocalin 2 (LCN2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Western Blotting (WB)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 21-198, His10, C-Term

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determine by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: BC50 ≥ 1.2 μM, by dose dependent binding of the protein with [ Fe3+(DHBA)3] complex.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for three months, or at -70°C for six months.

Target Details

Target: Lipocalin 2 (LCN2)

Alternative Name: NGAL

Background: NGAL, also known as lipocalin 2, LCN2, and siderocalin, is a small molecule composed of 178 amino acids. It belongs to the lipocalin superfamily of 20 structurally related secreted proteins. Members of the lipocalin family share a highly conserved fold with an eight stranded antiparallel β barrel, and act as a transporter, carrying small molecules to specific cells. NGAL occurs predominantly in a monomeric form, with a small percentage occurring as a dimer or trimer. It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils. It is a critical component of innate immunity because its ability to bind iron-siderophore complexes limits bacterial growth. Human NGAL is expressed at very low levels in human tissues, including kidney, trachea, lungs, stomach, and colon, and its expression increases greatly in the presence of inflammation and injured epithelia. In addition to its role in innate immunity, it is a promising biomarker for acute kidney injury that is beginning to be used in clinical practice in addition to research studies.

Molecular Weight: The 193 amino acid recombinant protein has a predicted molecular mass of approximately 22 kDa. The DTT-reduced and non-reduced protein migrate at approximately 24-28 and 23-26 kDa by SDS-PAGE respectively. The N-terminal amino acid is blocked.

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis