CCL19 Protein (AA 26-107)

Catalog No. ABIN2666783

This Recombinant CCL19 protein is expressed in HEK-293 Cells.

Quick Overview for CCL19 Protein (AA 26-107) (ABIN2666783)

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: Western Blotting (WB)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 26-107

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-hCCR7 transfectants in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Alternative Name: CCL19

Background: CCL19 is a CC chemokine expressed in lymph nodes. CCL19 is only 32 % identical to CCL21 at the amino acid level, nevertheless, these chemokines bind to the same receptor: CCR7. CCL19 is not expressed in high endothelial venules (HEV) as CCL21, but CCL19 is transcytosed in HEV. It has been described that CCL19 binds to CCX-CKR and CRAM-B receptors. These two receptors are members of the non classical chemokine receptor family which, in addition, includes D6, DARC, and CXCR7. CCX-CKR has been suggested to be a scavenging receptor for CCL19, and CRAM-B receptor might be responsible for the transcytosis in HEV for CCL19. In addition, CRAM recycles constitutively, and can internalize CCL19 within a few minutes, suggesting an implication in the regulation of immune responses and homing processes.

Molecular Weight: The 84 amino acid recombinant protein has a predicted molecular mass of approximately 9.3 kDa. The DTT-reduced and non-reduced protein migrate from 12-20 kDa by SDS-PAGE. The N-terminal amino acid is Gly.

Pathways: Positive Regulation of Immune Effector Process