IL-25 Protein (AA 17-169)

Catalog No. ABIN2666841

Recombinant IL-25 protein expressed in Insect Cells.

Quick Overview for IL-25 Protein (AA 17-169) (ABIN2666841)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Insect Cells

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 17-169

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Mouse IL-25 binds to recombinant mouse IL-17BR/Fc chimera in a functional ELISA.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in 20 mM NaHPO4 pH 6.0, 0.5 M NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL-25

Background: IL-25 (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F, and IL-17A/F. The primary sequence homology differs between family members, with mouse IL-17A and IL-17F having the highest homology (49 % ), while mouse IL-17A and IL-25 have the lowest (28 %). IL-25 induces gene expression of IL-4, IL-5, and IL-13 in multiple tissues, and this induction results in T helper 2 (TH2)-type immune responses, this is characterized by an increase in serum IgE levels, pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, an increase in mucus production, epithelial cell hyperplasia, and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.

Molecular Weight: The 153 amino acid recombinant protein has a predicted molecular mass of 17.6 kDa. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 20 kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer,