CCL20 Protein (Ala27, Met96)

Catalog No. ABIN2666889

This Recombinant CCL20 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL20 Protein (Ala27, Met96) (ABIN2666889)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: Ala27, Met96

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract BaF3-hCCR6 transfectants in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CCL20

Background: During infections, CCL20 expression is stimulated in epithelial cells, and immature CCR6+ DC are recruited. Thereafter, CCR6 is downregulated during dendritic cell (DC) maturation, and these migrate to the lymphoid node under the expression of CCR7. CCL20 is the only ligand for CCR6. Nevertheless, mouse and human β-defensin-1 and -2, small cationic antimicrobial peptides, bind and activate CCR6. Contrarily, these β-defensins have lower affinities for CCR6 than CCL20. Also, human CCL20 has potent antimicrobial activity. CCL20 and CCR6 play an important role in the pathogenesis of encephalomyelitis and psoriasis (chronic inflammatory skin disorder) in animal models. CCL20 is a chemokine abundant in psoriatic plaques characterized by epidermal hyperproliferation and immunocyte infiltration. CCL20 production from keratinocytes could be an initial trigger for Th17 activation and a key component in maintaining the self-perpetuating cycle of Th17 activation in psoriasis.

Molecular Weight: The 70 amino acid recombinant protein has a predicted molecular mass of approximately 8.0 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein at approximately 13 kDa by SDS-PAGE. The N-terminal amino acid is Ala.

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection