Interleukin 17a Protein (AA 26-158)

Catalog No. ABIN2667468

Recombinant Interleukin 17a protein expressed in Escherichia coli (E. coli).

Quick Overview for Interleukin 17a Protein (AA 26-158) (ABIN2667468)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 26-158

Sterility: 0.22 μm filtered

Endotoxin Level:

Endotoxin level is <0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: The ED50 is 0.25 - 1 ng/ml, corresponding to a specific activity 1.0 - 4 x 106 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of IL-6.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL-17A

Background: IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8. IL-17A shares 58 % homology with an open reading frame of the T-lymphotropic Herpes virus Samirii virus (viral IL-17). IL-17A belongs to a family of cytokines, which has five members, designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4 T cells, Th17 cells. This lineage selectively produces proinflammatory cytokines including IL-17, IL-17F, IL-21, and IL-22. In the mouse, the differentiation of this new lineage is initiated by TGFbeta-1 and IL-6 or IL-21, which activates Stat3 and induces the expression of the transcription factor retinoic acid-related orphan receptors (RORγt and RORα). IL-23, which also activates Stat3, serves to maintain Th17 cells lineage.

Molecular Weight: The 133 amino acid recombinant protein has a predicted molecular mass of 14,978 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer,