IL-25 Protein (AA 33-177)

Catalog No. ABIN2667478

This Recombinant IL-25 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-25 Protein (AA 33-177) (ABIN2667478)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Product Details

Protein Characteristics: AA 33-177

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Determined by its ability to induce IL-8 in human PBMCs using a concentration range of 10 - 100 ng.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL-17E

Background: IL-17E, also known as IL-25, shares the least structural homology with other IL-17 family members and is also functionally distinct from other IL-17 familiy members. IL-17E is primarily involved in type 2 immune response. IL-17E can induce Th2 cytokines, including IL-4, IL-5, IL-9 and IL-13, in multiple tissues. IL-17E can also inhibit pro-inflammatory Th1 and Th17 cytokine response. It has been well established that IL-17E plays an important role in allergy and asthma. Blocking IL-25 has been a therapeutic option. IL-25 is also required for protection against gastrointestinal helminth infections. IL-17E-deficient mice exihibit impaired Th2 immune response. Exclusion of helminth parasites was delayed in IL-17E-deficient mice. Recently, the potential neuroprotective effect of IL-17E is also being explored. IL-17E can regulate the development of autoimmune inflammation mediated by IL-17-prodcuing T cells. IL-17E-deficient mice are highly susceptible to experimential autoimmune encephalomyelitis. Interestingly, in vivo antitumor activity of IL-17E has also been reported.

Molecular Weight: The 145 amino acid recombinant protein has a predicted molecular mass of approximately 17 kDa. Recombinant human IL-17 is a disulfide-linked homodimer (~34 kDa). The predicted N-terminal amino acid is Met.