Annexin V Protein (Alexa Fluor 647)
Quick Overview for Annexin V Protein (Alexa Fluor 647) (ABIN2669252)
Target
See all Annexin V (ANXA5) ProteinsOrigin
Source
Application
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Purification tag / Conjugate
- This Annexin V protein is labelled with Alexa Fluor 647.
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Characteristics
- Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells.Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution (cat. no. 420403/420404) or Propidium Iodide Solution (cat. no. 421301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.
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Purification
- The purified protein was conjugated with Alexa Fluor® 647 under optimal conditions.
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Components
- Alexa Fluor 647 Annexin V, A647
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Annexin A5 (ANXA5) (AA 2-320) protein
ANXA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Annexin A5 (ANXA5) protein (FITC)ANXA5 Origin: Human Host: Bacteria Recombinant > 99 % (SDS-PAGE) FACS
Annexin A5 (ANXA5) (AA 1-319) protein (His-EGFP Tag)ANXA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Annexin A5 (ANXA5) (AA 1-320) protein (GST tag)ANXA5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Annexin A5 (ANXA5) (AA 1-320) proteinANXA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Buffer
- Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Protect from prolonged exposure to light. Do not freeze.
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Storage
- 4 °C
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Storage Comment
- The Annexin V solution should be stored undiluted between 2°C and 8°C.
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- Annexin V (ANXA5) (Annexin A5 (ANXA5))
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Alternative Name
- Annexin V
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Pathways
- Apoptosis
Target
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