KDM4A Protein (DYKDDDDK Tag)

Catalog No. ABIN2669705

This Recombinant KDM4A protein is expressed in Baculovirus.

Quick Overview for KDM4A Protein (DYKDDDDK Tag) (ABIN2669705)

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Product Details

Purification tag / Conjugate: This KDM4A protein is labelled with DYKDDDDK Tag.

Characteristics: Recombinant JMJD2A / KDM4A (accession number NP_055478.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 125.3 kDa.

Application Details

Application Notes: Recombinant JMJD2A / KDM4A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 and H3K36me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JMJD2A / KDM4A protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Alternative Name: JMJD2A / KDM4A

Background: KDM4A (lysine (K)-specific demethylase 4A), also known as JMJD2A (Jumonji Domain Containing 2A) is a nuclear protein that functions as a trimethylation-specific histone demethylase that preferentially demethylates trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated form. KDM4A has no activity for mono- and dimethylated H3K9 and H3K36. KDM4A functions as a transcriptional repressor, participating in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. An additional KDM4A isoform, KDM4A Isoform 2, that lacks the N-terminal demethylase domain is crucial for muscle differentiation in promoting transcriptional activation of the MyoG gene by directing the removal of repressive chromatin marks at its promoter.

Molecular Weight: 125.3 kDa

Pathways: Warburg Effect