Cortactin Protein (CTTN) (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2712518

Product Details

Target: Cortactin (CTTN)

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Cortactin protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Cortactin (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Cortés-Vicente, Gallardo, Martínez, Díaz-Manera, Querol, Rojas-García, Illa: "Clinical Characteristics of Patients With Double-Seronegative Myasthenia Gravis and Antibodies to Cortactin." in: JAMA neurology, Vol. 73, Issue 9, pp. 1099-104, (2016) (PubMed).

Labrador-Horrillo, Martínez, Selva-OCallaghan, Trallero-Araguás, Grau-Junyent, Vilardell-Tarrés, Juarez: "Identification of a novel myositis-associated antibody directed against cortactin." in: Autoimmunity reviews, Vol. 13, Issue 10, pp. 1008-12, (2014) (PubMed).

Gallardo, Martínez-Hernández, Titulaer, Huijbers, Martínez, Ramos, Querol, Díaz-Manera, Rojas-García, Hayworth, Verschuuren, Balice-Gordon, Dalmau, Illa: "Cortactin autoantibodies in myasthenia gravis." in: Autoimmunity reviews, Vol. 13, Issue 10, pp. 1003-7, (2014) (PubMed).

Wang, Henry, Distefano, Wang, Räikkönen, Mönkkönen, Tanaka, Morita: "Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human V?2V?2 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 3, pp. 1029-42, (2013) (PubMed).

Llanos, Royer, Lu, Bergamaschi, Lee, Lu: "Inhibitory member of the apoptosis-stimulating proteins of the p53 family (iASPP) interacts with protein phosphatase 1 via a noncanonical binding motif." in: The Journal of biological chemistry, Vol. 286, Issue 50, pp. 43039-44, (2011) (PubMed).

Target Details

Target: Cortactin (CTTN)

Alternative Name: Cortactin (CTTN Products)

Synonyms: EMS1 Protein, 1110020L01Rik Protein, Ems1 Protein, Cttnb Protein, CG3637 Protein, Dmel\CG3637 Protein, cortactin Protein, cttn Protein, CTTN1 Protein, P85.25 Protein, Cttn Protein, ems1 Protein, CTTN Protein, cortactin Protein, CG3637 gene product from transcript CG3637-RD Protein, cortactin S homeolog Protein, cortactin L homeolog Protein, src substrate cortactin Protein, Src substrate cortactin Protein, CTTN Protein, Cttn Protein, Cortactin Protein, cttn.S Protein, cttn.L Protein, cttn Protein, CpipJ_CPIJ006351 Protein, Bm1_57220 Protein, LOC100533277 Protein

Background: This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Molecular Weight: 57.3 kDa

NCBI Accession: NP_612632

Pathways: MAPK Signaling