Cortactin Protein (CTTN) (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2712518

Product Details

Target: Cortactin (CTTN)

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Cortactin protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Cortactin (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Cortés-Vicente, Gallardo, Martínez, Díaz-Manera, Querol, Rojas-García, Illa: "Clinical Characteristics of Patients With Double-Seronegative Myasthenia Gravis and Antibodies to Cortactin." in: JAMA neurology, Vol. 73, Issue 9, pp. 1099-104, (2016) (PubMed).

Labrador-Horrillo, Martínez, Selva-OCallaghan, Trallero-Araguás, Grau-Junyent, Vilardell-Tarrés, Juarez: "Identification of a novel myositis-associated antibody directed against cortactin." in: Autoimmunity reviews, Vol. 13, Issue 10, pp. 1008-12, (2014) (PubMed).

Gallardo, Martínez-Hernández, Titulaer, Huijbers, Martínez, Ramos, Querol, Díaz-Manera, Rojas-García, Hayworth, Verschuuren, Balice-Gordon, Dalmau, Illa: "Cortactin autoantibodies in myasthenia gravis." in: Autoimmunity reviews, Vol. 13, Issue 10, pp. 1003-7, (2014) (PubMed).

Wang, Henry, Distefano, Wang, Räikkönen, Mönkkönen, Tanaka, Morita: "Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human V?2V?2 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 3, pp. 1029-42, (2013) (PubMed).

Llanos, Royer, Lu, Bergamaschi, Lee, Lu: "Inhibitory member of the apoptosis-stimulating proteins of the p53 family (iASPP) interacts with protein phosphatase 1 via a noncanonical binding motif." in: The Journal of biological chemistry, Vol. 286, Issue 50, pp. 43039-44, (2011) (PubMed).

Target Details

Target: Cortactin (CTTN)

Alternative Name: Cortactin (CTTN Products)

Background: This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Molecular Weight: 57.3 kDa

NCBI Accession: NP_612632

Pathways: MAPK Signaling